%0 Journal Article %T Coxiella burnetii in slaughterhouses in Brazil: A public health concern %+ Universidade Estadual Paulista Júlio de Mesquita Filho = São Paulo State University (UNESP) %+ Universidade Estadual do Maranhão = State University of Maranhão (UEMA) %+ Universidade de São Paulo = University of São Paulo (USP) %+ Laboratoire de Sophia Antipolis %A Mioni, Mateus de Souza Ribeiro %A Costa, Francisco Borges %A Ribeiro, Bruna Letícia Devidé %A Teixeira, Wanderson Sirley Reis %A Pelicia, Vanessa Cristina %A Labruna, Marcelo Bahia %A Rousset, Élodie %A Sidi-Boumedine, Karim %A Thiéry, Richard %A Megid, Jane %Z MSR Mioni: The General Program for International Cooperation (PGCI) from the Coordination of Improvement of Higher-Level Personnel (CAPES) provided the scholarship number 88887.137563/2017-00, and scholarship 88882.180556/2018-01 was provided by the Program of Academic Excellence (PROEX) from the Coordination of Improvement of Higher-Level Personnel (CAPES) (https://www.capes.gov.br/). FAPESP, scholarship 2019/05300-9 (http://www.fapesp.br/). %< avec comité de lecture %Z LNR Fièvre Q %@ 1932-6203 %J PLoS ONE %I Public Library of Science %V 15 %N 10 %P e0241246 %8 2020 %D 2020 %R 10.1371/journal.pone.0241246 %M 33125388 %K Q fever %Z Life Sciences [q-bio]/Microbiology and Parasitology/VirologyJournal articles %X Q fever is an important zoonosis, yet it is often neglected and can present large outbreaks, as observed in the Netherlands. In the past few years, cases of Q fever have been described in Brazil; however, the epidemiological situation of Q fever in ruminants, the main reservoir of the pathogen, is unknown in this country. Our study aimed to estimate the prevalence of C. burnetii in cattle sent to slaughterhouses using an immunofluorescence assay (IFA) and quantitative real-time PCR (qPCR). From 1515 cattle serum samples collected from nine slaughterhouses, 23.8% (360/1515) were serologically positive by IFA (cutoff titer>1:64), indicating past or recent exposure to C. burnetii infection. Among the 54 cities sampled during the study, 83.3% (45/54) had at least one seropositive animal. Subsequently, all seropositive samples were submitted to qPCR for C. burnetiiDNA, and 12.2% (44/360) of the sera were qPCR positive, which indicates bacteremia and suggests active or recent infection. The results highlight the risk for abattoir workers that results from exposure to contaminated aerosols produced during slaughter procedures. Moreover, the heat maps that were construction from the positive samples demonstrate the widespread distribution of C. burnetii in the State of São Paulo, Brazil and denotes the need for surveillance and preventive measures to reduce the prevalence in cattle. %G English %L anses-03626324 %U https://anses.hal.science/anses-03626324 %~ ANSES %~ AGREENIUM