%0 Journal Article %T Assessment of the mercury-selenium antagonism in rainbow trout fish %+ Laboratoire de sécurité des aliments de Maisons-Alfort (LSAl) %+ Universidade de Lisboa = University of Lisbon (ULISBOA) %+ Instituto Nacional de Saùde Dr Ricardo Jorge [Portugal] (INSA) %+ Laboratoire de Ploufragan-Plouzané-Niort [ANSES] %+ Direction de la Stratégie et des Programmes (DSP) %A Ribeiro, Mariana %A Zephyr, N. %A Silva, J.A.L. %A Danion, Morgane %A Guérin, Thierry %A Castanheira, I. %A Leufroy, Axelle %A Jitaru, Petru %Z This project (MERSEL-FISH) was funded by ANSES and INSA. %< avec comité de lecture %@ 0045-6535 %J Chemosphere %I Elsevier %V 286 %N Pt 2 %P 131749 %8 2022 %D 2022 %R 10.1016/j.chemosphere.2021.131749 %M 34426140 %K Antagonism %K Dietary exposure %K ICP-MS %K Mercury %K Rainbow trout fish %K Selenium %Z Life Sciences [q-bio]Journal articles %X This study aims at the assessment of mercury (Hg)-selenium (Se) antagonism in fish. For this purpose, rainbow trout fish (Oncorhynchus mykiss) were exposed to methylmercury (MeHg) under controlled conditions, in the presence or absence of selenomethionine (SeMet) using an in-house prepared diet (enriched with MeHg and SeMet at 0.2 μg/g and 5.0 μg/g, respectively). The total duration of the exposure study was 3 months. Fish was sampled and analysed for total Se (SeT) and total mercury (HgT) content after 1, 2 and 3 months of exposure. Six feeding protocols were compared, depending on the exposure type: (i) no MeHg nor SeMet exposure (control group); (ii) exposure to SeMet solely; (iii) exposure to MeHg solely; (iv) exposure to both MeHg and SeMet; (v) exposure first to MeHg during 1 month and then to SeMet during 2 months and (vi), exposure to SeMet during 1 month and then to MeHg for 2 months. The levels of SeT and HgT in the fish (control and supplemented with MeHg/SeMet) were measured by inductively coupled plasma-mass spectrometry (ICPMS). Steadily (linear) bioaccumulation of MeHg in the fish muscle occurred when the fish were exposed individually to this species during the period. The bioaccumulation of MeHg is diminished when the fish are firstly exposed to SeMet and then to MeHg, hence indicating the MeHg detoxification due to SeMet supplementation. %G English %2 https://anses.hal.science/anses-03651307/document %2 https://anses.hal.science/anses-03651307/file/S0045653521022219.pdf %L anses-03651307 %U https://anses.hal.science/anses-03651307 %~ ANSES %~ AGREENIUM %~ INRAE %~ ELSEVIER %~ TEST3-HALCNRS %~ EMERGIN