Further spread of perch rhabdovirus on European percid farms - Anses - Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail Access content directly
Journal Articles Journal of Fish Diseases Year : 2022

Further spread of perch rhabdovirus on European percid farms

Abstract

Variants of perch rhabdovirus (PRV) circulate across European percid farms via the fish trade. To trace their circulation, they are usually isolated by cell culture and subsequently identified genetically by sequencing partial or complete genes. Here, a newly developed nested PCR-based method was used to amplify and sequence the complete N and P genes directly from clinical samples obtained during an outbreak on a farm as well as from four batches of fish sampled from two other farms in another country. In an attempt to trace the origin of the five detected viruses, their N and P sequences were concatenated and compared with related viruses. One virus found in pike-perch was highly related to a virus isolated in 2016 in Belgium. Two other viruses detected on a single farm were distinct from one another, with one being almost identical to another virus isolated in 2016 in Belgium and the other being more closely related to a subgroup with different origins, France and Belgium. Two other viruses found in perch from a third farm were identical and were more related to a subgroup of viruses isolated in France. Identifying variants by a direct PCR approach will help to prevent further dissemination in farms.
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anses-03967826 , version 1 (01-02-2023)

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Laurane Pallandre, Heike Schmidt-Posthaus, Françoise Pozet, Mélanie Rupp, Elodie Cristina, et al.. Further spread of perch rhabdovirus on European percid farms. Journal of Fish Diseases, 2022, 45 (12), pp.1831-1837. ⟨10.1111/jfd.13705⟩. ⟨anses-03967826⟩

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