%0 Journal Article %T Penicillin-Binding Protein Gene Alterations in Streptococcus uberis Isolates Presenting Decreased Susceptibility to Penicillin %+ Unité Antibiorésistance et Virulence Bactériennes (AVB) %+ Department of Fundamental Microbiology [Lausanne] %A Haenni, Marisa %A Galofaro, Laure %A Ythier, Mathilde %A Giddey, Marlyse %A Majcherczyk, Paul, A %A Moreillon, Philippe %A Madec, Jean-Yves %< avec comité de lecture %@ 0066-4804 %J Antimicrobial Agents and Chemotherapy %I American Society for Microbiology %V 54 %N 3 %P 1140 - 1145 %8 2010-03 %D 2010 %R 10.1128/aac.00915-09 %Z Life Sciences [q-bio]/Microbiology and Parasitology/BacteriologyJournal articles %X Streptococcus uberis is an environmental pathogen commonly causing bovine mastitis, an infection that is generally treated with penicillin G. No field case of true penicillin-resistant S. uberis (MIC > 16 mg/liter) has been described yet, but isolates presenting decreased susceptibility (MIC of 0.25 to 0.5 mg/liter) to this drug are regularly reported to our laboratory. In this study, we demonstrated that S. uberis can readily develop penicillin resistance in laboratory-evolved mutants. The molecular mechanism of resistance (acquisition of mutations in penicillin-binding protein 1A [PBP1A], PBP2B, and PBP2X) was generally similar to that of all other penicillin-resistant streptococci described so far. In addition, it was also specific to S. uberis in that independent resistant mutants carried a unique set of seven consensus mutations, of which only one (Q 554 E in PBP2X) was commonly found in other streptococci. In parallel, independent isolates from bovine mastitis with different geographical origins (France, Holland, and Switzerland) and presenting a decreased susceptibility to penicillin were characterized. No mosaic PBPs were detected, but they all presented mutations identical to the one found in the laboratory-evolved mutants. This indicates that penicillin resistance development in S. uberis might follow a stringent pathway that would explain, in addition to the ecological niche of this pathogen, why naturally occurring resistances are still rare. In addition, this study shows that there is a reservoir of mutated PBPs in animals, which might be exchanged with other streptococci, such as Streptococcus agalactiae, that could potentially be transmitted to humans. %G English %2 https://anses.hal.science/anses-04018322/document %2 https://anses.hal.science/anses-04018322/file/Haenni_MutationsResistancePBPuberis_AAC2010.pdf %L anses-04018322 %U https://anses.hal.science/anses-04018322 %~ ANSES %~ AGREENIUM