Detection and antimicrobial susceptibility of Plesiomonas shigelloides in a French River - Anses - Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail Accéder directement au contenu
Communication Dans Un Congrès Année : 2023

Detection and antimicrobial susceptibility of Plesiomonas shigelloides in a French River

Résumé

As part of a research project to investigate the spread of antimicrobial resistance (AMR), bacteriological monitoring survey was carried out along a river, where two fish farms are located one near the source the other at the mouth. For 17 months, every two weeks, water samples were collected upstream and downstream the two fish farms and biofilm samples were collected in a same fishpond per farm. 144 water and the 72 biofilm samples were cultured on TBX and TSA at 37°C for 48°h. The collection is composed of 3,088 isolates, 957 coming from biofilm (248 from TBX, 709 from TSA) and 2,131 from water samples (691 from TBX, 1,440 from TSA). A total of 71 isolates of Plesiomonas shighelloides were identified by Maldi-Tof and confirmed by PCR. Plesiomonas was detected in 41.6% (15/36) of the sampling campaigns in the water samples and/or in the biofilm samples. Detection of Plesiomonas was unsuccessful in samples collected between October and May. Most of the isolates were collected from the source of the river (n=50) and from TBX (86%, n=61). Among the isolates collected on TBX, the prevalence of Plesiomonas is higher in biofilm than in water, 21.4% and 1.15% respectively. On a selection of 36 isolates (one per sample, per date and per culture media) genetic diversity by ERIC-PCR, and antimicrobial susceptibility by agar diffusion were performed. A panel of 31 agents including nine classes was tested. Clinical breakpoint defined by CLSI for Enterobacterial (M100 ed 31) were applied. Based on the ERIC-PCR, the isolates are divided into four groups with one profile (profil A) representing 75% of isolates. 25 out of 26 isolates of this profile were isolated from TBX. Thus, we can suppose that the media of culture affected the diversity of the isolates. Among the ten other isolates, all collected from TSA, the profile B is dominant with seven isolates. No link between geographical or origin of the sample and profile was observed. Nevertheless, ERIC-PCR is a methodology for a first screening; PGFE will allowed a better discrimination. Using available clinical breakpoint, the 36 isolates tested were all susceptible to quinolone. Among beta-lactam class, all isolates were susceptible to third generation cephalosporins and carbapenems. At the opposite all isolates were categorized as resistant (n=35) or intermediate (n=1) to ampicillin. We could suspected an initiate resistance to this molecule but not to the penicillin class. Indeed, only one isolate was categorized as intermediate to piperacillin. 88.9% of the isolates were intermediate (n=31) or resistant (n=1) to kanamycin, three of them were intermediate to amikacin too; and one isolate is also intermediate to gentamycin and resistant to amikacin. But all isolates are susceptible to netilmicin. Twenty-one isolates presented the same multidrug resistance profile: tetracycline – SXT – Trimethoprim – ampicillin and intermediate to kanamycin. These isolates excepted one belong to ERIC-PCR profile A and were collected from TBX. Detection of resistance gene by qPCR system (i) was performed on ten of them. They all harbored resistance gene associated with resistance to sulfonamide (sul1, sul2), to trimethoprim (dfrA12). This in agreement with phenotypic results and could be linked to the presence of integron class 1. These first results raise the question of the possible role of Plesiomonas in the dissemination of antibiotic resistance in water and therefore in fish. Thanks to the complementarity of the research teams, an in-depth study of this collection (serotyping, genetic diversity, etc.) will be able to continue.
Fichier non déposé

Dates et versions

anses-04168523 , version 1 (21-07-2023)

Identifiants

  • HAL Id : anses-04168523 , version 1

Citer

Karolina Ucieklak, Laetitia Le Devendec, Eric Jouy, Emeline Larvor, Jens Andre Hammerl, et al.. Detection and antimicrobial susceptibility of Plesiomonas shigelloides in a French River. 13. International Symposium on Aeromonas and Plesiomonas (ISAP), Jun 2023, Wroclaw, Poland. ⟨anses-04168523⟩

Collections

ANSES
51 Consultations
0 Téléchargements

Partager

Gmail Mastodon Facebook X LinkedIn More