CTX-M-15/27-positive Escherichia coli and VIM-2-producing Pseudomonas putida in free-living pigeons (Columba livia) in Tunisia - Anses - Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail Accéder directement au contenu
Article Dans Une Revue Journal of Global Antimicrobial Resistance Année : 2023

CTX-M-15/27-positive Escherichia coli and VIM-2-producing Pseudomonas putida in free-living pigeons (Columba livia) in Tunisia

Résumé

Objectives: Wild birds are vectors of antimicrobial resistance. Birds living in close contact with humans or other animals, like feral pigeons (Columba livia), might be especially prone to acquire resistance genes such as those encoding extended-spectrum beta-lactamases (ESBLs) and carbapenemases. Methods: Cloacal samples (n = 206) of free-living feral pigeons (C. livia) were collected in Sousse and Monastir, Tunisia. Antimicrobial susceptibility profiles were determined by disc-diffusion, and resistant isolates were short-and long-read whole-genome sequenced. Sequence analysis was performed using tools of the Centre for Genomic Epidemiology, and Phylogenetic analysis was performed based on the core-genome MLST. Results: Fourteen (14/206, 6.8%) pigeons harboured Enterobacterales resistant to last-generations cephalosporins, of which 10 were CTX-M-15-or CTX-M-27-producers, while two (1.0%) carried a VIM-2producing Pseudomonas putida. Positive pigeons lived on four different livestock farms. Three STs (ST206, ST5584, ST8149) were identified among E. coli , of which ST5584 and ST8149 were found in two different farms. Genetic diversity was also observed in Enterobacter cloacae and P. putida isolates. The bla CTX-M-27 genes were chromosomally encoded, while the bla CTX-M-15 genes were carried on highly similar IncF/F-:A-:B53 plasmids. The bla VIM-2 gene was located on a class 1 integron co-harbouring several resistance genes. Conclusion: Pigeons living on livestock farms carried clinically important resistance genes encoding ESBLs and carbapenemases. Our results evidenced that both clonal (ST8149 and ST5584) and plasmidic (IncF/F-:A-:B53) transfers played a role in the spread of resistance genes among pigeons. Further studies are needed to identify factors favouring the transfer and persistence of resistance genes within the pigeon communities.

Domaines

Bactériologie
Fichier principal
Vignette du fichier
Souguir_Pigeons_JGAR2024.pdf (1.45 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Licence : CC BY ND - Paternité - Pas de modifications

Dates et versions

anses-04387345 , version 1 (11-01-2024)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

Identifiants

Citer

Meriem Souguir, Pierre Châtre, Antoine Drapeau, Sana Azaiez, Imen Hmidi, et al.. CTX-M-15/27-positive Escherichia coli and VIM-2-producing Pseudomonas putida in free-living pigeons (Columba livia) in Tunisia. Journal of Global Antimicrobial Resistance, 2023, 36, pp.70-75. ⟨10.1016/j.jgar.2023.12.013⟩. ⟨anses-04387345⟩

Collections

ANSES
10 Consultations
5 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More