Epidemiological cut-off values for non-O1/ non-O139 Vibrio cholerae disc diffusion data generated by standardised methods - Anses - Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail Accéder directement au contenu
Article Dans Une Revue Diseases of Aquatic Organisms Année : 2023

Epidemiological cut-off values for non-O1/ non-O139 Vibrio cholerae disc diffusion data generated by standardised methods

Résumé

This work generates the data needed to set epidemiological cutoff values for discdiffusion zone measurements of Vibrio cholerae. The susceptibility of 147 European isolates of non-O1/non-O139 V. cholerae to 19 antibiotics was established using a standardised disc diffusion method which specified incubation of Mueller Hinton agar plates at 35°C. Epidemiological cutoff values were calculated by analysis of the zone size data with the statistically based normalised resistance interpretation method. Cutoff values for 17 agents were calculated by analysis of the aggregated data from all 4 laboratories participating in this study. The cutoff values calculated were ≥ 18 mm for amoxicillin/clavulanate, ≥ 18 mm for amikacin, ≥ 19 mm for ampicillin, ≥ 27 mm for cefepime, ≥ 31 mm for cefotaxime, ≥ 24 mm for ceftazidime, ≥ 24 mm for chloramphenicol, ≥ 31 mm for ciprofloxacin, ≥ 16 mm for erythromycin, ≥ 27 mm for florfenicol, ≥ 16 mm for gentamicin, ≥ 23 mm for imipenem, ≥ 25 mm for meropenem, ≥ 29 mm for nalidixic acid, ≥ 28 mm for norfloxacin, ≥ 13 mm for streptomycin and ≥ 23 mm for tetracycline. For the other 2 agents the data from 1 laboratory was excluded from the censored aggregation because the data from that laboratory was considered excessively imprecise. The cutoff values for these 2 agents calculated for the aggregation of the data from 3 laboratories were ≥ 23 mm for trimethoprim and ≥ 24 mm for trimethoprim/sulfamethoxazole. These zone size data will be submitted to the Clinical Laboratory Standards Institute (CLSI) and European Committee for Antimicrobial Susceptibility Testing (EUCAST) for their consideration in setting international consensus epidemiological cutoff values for non O1/non-O139 V. cholerae.
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anses-04395805 , version 1 (15-01-2024)

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Peter Smith, Laëtitia Le Devendec, Eric Jouy, Emeline Larvor, Jean Lesne, et al.. Epidemiological cut-off values for non-O1/ non-O139 Vibrio cholerae disc diffusion data generated by standardised methods. Diseases of Aquatic Organisms, 2023, 156, pp.115 - 121. ⟨10.3354/dao03766⟩. ⟨anses-04395805⟩
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