Field evaluation of a safe and cost-effective shipment of FMDV suspected samples to diagnostic laboratories using lateral flow devices - Anses - Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail Accéder directement au contenu
Communication Dans Un Congrès Année : 2023

Field evaluation of a safe and cost-effective shipment of FMDV suspected samples to diagnostic laboratories using lateral flow devices

Aurore Romey
Abdulnaci Bulut
  • Fonction : Auteur
Syed Jamal
Salman Khan
Muhammad Ishaq
Michael Eschbaumer
  • Fonction : Auteur
  • PersonId : 1006303
Graham J. Belsham
  • Fonction : Auteur
  • PersonId : 1019759
Anthony Relmy
  • Fonction : Auteur
  • PersonId : 1075108
Mathilde Gondard
Souheyla Benfrid
Stéphan Zientara

Résumé

Introduction Foot-and-mouth disease virus (FMDV) causes a highly contagious vesicular disease in livestock with serious consequences for international trade (1,2). The control of FMD requires the identification of circulating strains in affected areas to implement effective control measures. However, restrictive regulations and high costs for shipping clinical samples to reference laboratories remain major obstacles. In our laboratory, we developed a cost-effective and safe protocol for shipment of FMDV samples based on the inactivation of the virus on lateral flow devices (LFD) (3). This method allows molecular detection and typing of FMDV and rescue of live virus after RNA transfection into cells. The present study further evaluates this protocol on clinical samples freshly collected in endemic countries. Methods In Nigeria, Turkey and Pakistan, epithelium or vesicular fluid samples were collected on FMD-suspected clinical cases and tested in the field for FMD using LFD. LFDs were chemically inactivated or not, directly in the field, then submitted to reference laboratories for further molecular detection and virus rescue. In parallel, the protocol was tested on vesicular fluid collected from cattle experimentally infected with FMDV. Results The safety of the inactivation protocol was confirmed on field samples, as well as on vesicular fluid collected from experimentally infected cattle. On inactivated LFDs, molecular diagnosis was successfully performed allowing VP1 sequencing and molecular characterisation of strain involved. Transfection of RNA extracted from inactivated LFDs ensured virus rescue for some samples allowing thus further characterization, as sequencing of complete genome. Conclusions This study demonstrates that this LFDs-based inactivation protocol, applicable directly in the field, is a safe way for dry-transportation of FMDV samples. It will substantially decrease the shipping cost, thus increasing field sample submission to contribute to a better FMD control.
Fichier non déposé

Dates et versions

anses-04426628 , version 1 (30-01-2024)

Licence

Paternité - Pas de modifications

Identifiants

  • HAL Id : anses-04426628 , version 1

Citer

Aurore Romey, Hussaini Ularamu, Abdulnaci Bulut, Syed Jamal, Salman Khan, et al.. Field evaluation of a safe and cost-effective shipment of FMDV suspected samples to diagnostic laboratories using lateral flow devices. ISWAVLD 2023 (International Symposium of the World Association of Veterinairy Laboratory Diagnosticians, ADILVA, ANSES, VetAgrosup, Jun 2023, Lyon, France. ⟨anses-04426628⟩
4 Consultations
0 Téléchargements

Partager

Gmail Facebook X LinkedIn More