New Structure of Aeromonas salmonicida O-Polysaccharide Isolated from Ill Farmed Fish - Anses - Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail
Article Dans Une Revue Microorganisms Année : 2024

New Structure of Aeromonas salmonicida O-Polysaccharide Isolated from Ill Farmed Fish

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The diversity of O-polysaccharides (O-antigens) among 28 Aeromonas salmonicida strains isolated from ill fish has been determined by using high-resolution magic angle spinning (HR MAS) NMR spectroscopy. The new O-polysaccharide has been identified in two isolates. This new structure was investigated by 1H and 13C NMR spectroscopy and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The following structure of the linear hexasaccharide repeating unit of A. salmonicida O-antigen has been established: →3)-α-L-Rhap-(1→3)-α-D-ManpNAc-(1→2)-β-D-Glcp-(1→3)-α-L-Rhap2OAc4OAc-(1→3)-β-D-ManpNAc-(1→3)-α-D-Glcp-(1→. This new A. salmonicida O-polysaccharide was detected among two isolates collected from trout and turbot fish in 2010 and 2011, respectively. Further investigations should be conducted to evaluate the distribution of this new O-polysaccharide among a larger collection of isolates, depending on their geographic origin, the species of fish, and the health status of the fish.
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anses-04693995 , version 1 (19-09-2024)

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Karolina Ucieklak, Sylwia Wojtys-Tekiel, Garance Leroy, Laëtitia Le Devendec, Sandrine Baron, et al.. New Structure of Aeromonas salmonicida O-Polysaccharide Isolated from Ill Farmed Fish. Microorganisms, 2024, 12 (8), pp.1575. ⟨10.3390/microorganisms12081575⟩. ⟨anses-04693995⟩
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