Communication Dans Un Congrès Année : 2024

SAFE AND COST-EFFECTIVE SHIPMENT OF FMDV SUSPECTED SAMPLES TO DIAGNOSTIC LABORATORIES: EVALUATION OF THE PROCESS ON LFD PRODUCED BY DIFFERENT MANUFACTORS

Résumé

Background/Introduction The control of FMD requires the identification of circulating strains in affected areas to implement effective control measures. However, restrictive regulations and high costs for shipping clinical samples to reference laboratories remain major obstacles. In our laboratory, we developed a cost-effective and safe protocol for shipment of FMDV samples based on the inactivation of the virus on lateral flow devices (LFD). This method allows molecular detection and typing of FMDV and rescue of live virus after RNA transfection into cells. This protocol has been further evaluated on clinical samples freshly collected in endemic countries. The present study aims to test the efficacy of this protocol on different LFD references available on the market. Material and methods Four different references of LFD have been used with FMDV strains, from the EURL collection, representative of the 6 major serotypes. Some of the new LFDs tested are designed to allow typing of strain involved in addition of FMDV detection. Sensitivity of LFDs tested has been firstly estimated. In a second step, the efficiency of the chemical inactivation process in a 0.2% citric acid bath during 15 minutes has been evaluated. Finally, detection of viral RNA after inactivation step has been checked. Results The first results obtained on the four new LFD references tested were not in line with the ones of previous studies (conducted on a no longer available reference). The chemical treatment applied was indeed not sufficient to systematically inactivate live FMDV on each LFD. After investigation, we concluded that the stability of citric acid powder from the date the bottle is opened is a critical step. Further analyses have been performed with a new batch of powder, with different concentrations of citric acid bath, and different time of incubation, in different containers, to finally show a real inactivation of live virus. Viral RNA was still detectable by rtRT-PCR. Discussion and conclusions This study confirms that this LFDs-based inactivation protocol, applicable directly in the field, is a safe way for room-temperature, dry-transportation of FMDV samples. However, application of this protocol on different LFD references need to be evaluated in BSL-3 laboratory before being applied in the field: testing on different FMDV strains with a range of viral titer.
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Dates et versions

anses-04805449 , version 1 (26-11-2024)

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  • HAL Id : anses-04805449 , version 1

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Aurore Romey, Efrem Alessandro Foglia, Anne-Laure Salomez, Anthony Relmy, Cindy Bernelin-Cottet, et al.. SAFE AND COST-EFFECTIVE SHIPMENT OF FMDV SUSPECTED SAMPLES TO DIAGNOSTIC LABORATORIES: EVALUATION OF THE PROCESS ON LFD PRODUCED BY DIFFERENT MANUFACTORS. EUFMD Open Session 2024, EuFMD, Oct 2024, Alcala de Henares (Madrid), Spain. ⟨anses-04805449⟩
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