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Journal Articles Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology Year : 2016

High throughput toxicity screening and intracellular detection of nanomaterial

Andrew R. Collins
  • Function : Author
Balasubramanyam Annangi,
  • Function : Author
Laura Rubio,
  • Function : Author
Ricard Marcos,
  • Function : Author
Marco Dorn,
  • Function : Author
Carolin Merker,
  • Function : Author
Irina Estrela-Lopis,
  • Function : Author
Roxana Cimpan, Mihaela
  • Function : Author
Mohamed Ibrahim,
  • Function : Author
Emil Cimpan,
  • Function : Author
Melanie Ostermann,
  • Function : Author
Alexander Sauter,
  • Function : Author
El Yamani, Naouale
  • Function : Author
Sergey Shaposhnikov,
  • Function : Author
Sylvie Chevillard,
  • Function : Author
Vincent Paget,
  • Function : Author
Romain Grall,
  • Function : Author
Jozo Delic,
  • Function : Author
Goñi De-Cerio, Felipe
  • Function : Author
Blanca Suarez-Merino,
  • Function : Author
Maria Fjellsbø, Lise
  • Function : Author
Runden Pran, Elise
  • Function : Author
Tana Brzicova,
  • Function : Author
Jan Topinka,
  • Function : Author
João Silva, Maria
  • Function : Author
E. Leite, P.
  • Function : Author
R. Ribeiro, A.
  • Function : Author
M. Granjeiro, J.
  • Function : Author
Roland Grafström,
  • Function : Author
Adriele Prina-Mello,
  • Function : Author
Maria Dusinska,
  • Function : Author
  • PersonId : 983588

Abstract

With the growing numbers of nanomaterials (NMs), there is a great demand for rapid and reliable ways of testing NM safety—preferably using in vitro approaches, to avoid the ethical dilemmas associated with animal research. Data are needed for developing intelligent testing strategies for risk assessment of NMs, based on grouping and read-across approaches. The adoption of high throughput screening (HTS) and high content analysis (HCA) for NM toxicity testing allows the testing of numerous materials at different concentrations and on different types of cells, reduces the effect of inter-experimental variation, and makes substantial savings in time and cost. HTS/HCA approaches facilitate the classification of key biological indicators of NM-cell interactions. Validation of in vitro HTS tests is required, taking account of relevance to in vivo results. HTS/HCA approaches are needed to assess dose- and time-dependent toxicity, allowing prediction of in vivo adverse effects. Several HTS/HCA methods are being validated and applied for NM testing in the FP7 project NANoREG, including Label-free cellular screening of NM uptake, HCA, High throughput flow cytometry, Impedance-based monitoring, Multiplex analysis of secreted products, and genotoxicity methods—namely High throughput comet assay, High throughput in vitro micronucleus assay, and γH2AX assay. There are several technical challenges with HTS/HCA for NM testing, as toxicity screening needs to be coupled with characterization of NMs in exposure medium prior to the test; possible interference of NMs with HTS/HCA techniques is another concern. Advantages and challenges of HTS/HCA approaches in NM safety are discussed. For further resources related to this article, please visit the WIREs website.

Domains

Toxicology

Dates and versions

anses-01331847 , version 1 (14-06-2016)

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Cite

Andrew R. Collins, Balasubramanyam Annangi,, Laura Rubio,, Ricard Marcos,, Marco Dorn,, et al.. High throughput toxicity screening and intracellular detection of nanomaterial. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology, 2016, ⟨10.1002/wnan.1413⟩. ⟨anses-01331847⟩

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