Reduced susceptibilities to biocides and resistance to antibiotics in food-associated bacteria following exposure to quaternary ammonium compounds - Anses - Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail Access content directly
Journal Articles Journal of Applied Microbiology Year : 2016

Reduced susceptibilities to biocides and resistance to antibiotics in food-associated bacteria following exposure to quaternary ammonium compounds

Delphine Méheust
Patricia Legrandois
  • Function : Author
  • PersonId : 996534
Bastien Fremaux
  • Function : Author
Carole Feurer
  • Function : Author
Alain Le Roux
  • Function : Author
Pierre Maris
  • Function : Author
  • PersonId : 904982

Abstract

Aims: Our aim was to assess the effects of step-wise exposure to didecyl dimethyl ammonium chloride (DDAC) on the antimicrobial (antibiotics and biocides) susceptibilities of food-associated bacterial strains. Methods and Results: Adaptive responses of bacterial strains were investigated by exposing the strains daily to increasing subinhibitory concentrations of DDAC for 7 days. Following adaptation to DDAC, a threefold increase in the minimum inhibitory concentration (MIC) values for this biocide was observed in 48% of the Escherichia coli and Listeria monocytogenes strains, and 3% of the Salmonella strains. Reduced susceptibility to other biocides was found with the most important increase in MIC for benzalkonium chloride (BC) and a commercial biocide formulation (Galox Horizon) containing DDAC and glutaraldehyde, for all species except Salmonella. Increase in antibiotic MIC values was more pronounced in E. coli in terms of antibiotic numbers and of magnitude (from 4- to 32-fold increase) and, to a lesser extent, in Salmonella strains. Most of these strains had acquired resistance to ampicillin, cefotaxime, ceftazidime, chloramphenicol and ciprofloxacin. Conclusions: The effects of exposure to DDAC on biocides and antibiotics susceptibilities depend upon the bacteria species. Significance and Impact of the Study: Extensive use of DDAC at subinhibitory concentrations may lead to the development of antibiotic-resistant bacteria and may represent a public health issue.
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anses-01419300 , version 1 (05-10-2023)

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Christophe Soumet, Delphine Méheust, Christine Pissavin, Patricia Legrandois, Bastien Fremaux, et al.. Reduced susceptibilities to biocides and resistance to antibiotics in food-associated bacteria following exposure to quaternary ammonium compounds. Journal of Applied Microbiology, 2016, 121 (5), pp.1275-1281. ⟨10.1111/jam.13247⟩. ⟨anses-01419300⟩

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