Reliability of Morphological and PCR Methods for the Official Diagnosis of Aethina tumida: A European Inter-Laboratory Comparison - Anses - Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail Access content directly
Conference Poster Year : 2022

Reliability of Morphological and PCR Methods for the Official Diagnosis of Aethina tumida: A European Inter-Laboratory Comparison

Abstract

The Small Hive Beetle (Aethina tumida Murray, 1867) is an invasive scavenger of honeybees. Originally endemic in sub-Saharan Africa, it is regulated internationally in order to preserve the areas still free from this species. To ensure the reliability of official diagnoses, the European Reference Laboratory for Bee Health organised an inter-laboratory comparison on the identification of A. tumida by morphology and real-time PCR, two types of methods used routinely to confirm outbreaks. Twenty-two National Reference Laboratories in Europe participated in the study and analysed 12 samples with adult coleopterans and insect larvae. The performance of the laboratories was evaluated in terms of sensitivity and specificity. Sensitivity was satisfactory for all the participants and both types of methods, thus fully meeting the diagnostic challenge of confirming all truly positive cases as positive. Two participants encountered specificity problems. For one, the anomaly was minor whereas, for the other, the issues concerned a larger number of results, especially real-time PCR, which probably were related to inexperience with this technique. The present comparison is, to our knowledge, the first organised at the international level on the official diagnosis of A. tumida. It demonstrated the reliability of official diagnosis, including the entire analytical process of A. tumida identification: from the first step of the analysis to the expression of opinions. The performed diagnostic tools, in parallel with field surveillance, are essential to managing A. tumida introduction in countries where its presence has not yet been detected and where early detection is crucial.
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Dates and versions

anses-03930154 , version 1 (09-01-2023)

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  • HAL Id : anses-03930154 , version 1

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Stéphanie Franco, Nicolas Cougoule, Amandine Tison, Aurélie del Cont, Cristina Gastaldi, et al.. Reliability of Morphological and PCR Methods for the Official Diagnosis of Aethina tumida: A European Inter-Laboratory Comparison. Eurbee9, 9th European Congress of Apidology, Sep 2022, Belgrade, Serbia. , 2022. ⟨anses-03930154⟩

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