Horsing Around: Escherichia coli ST1250 of Equine Origin Harboring Epidemic IncHI1/ST9 Plasmid with blaCTX-M-1 and an Operon for Short-Chain Fructooligosaccharide Metabolism - Anses - Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail Access content directly
Journal Articles Antimicrobial Agents and Chemotherapy Year : 2021

Horsing Around: Escherichia coli ST1250 of Equine Origin Harboring Epidemic IncHI1/ST9 Plasmid with blaCTX-M-1 and an Operon for Short-Chain Fructooligosaccharide Metabolism

Peter Damborg

Abstract

The relatedness of the equine-associated Escherichia coli strain ST1250and its single- and double-locus variants (ST1250-SLV/DLV), obtained from horsesin Europe, was studied by comparative genome analysis. A total of 54 isolates of E.coli ST1250 and ST1250-SLV/DLV from healthy and hospitalized horses acrossEurope (Czech Republic [n = 23], The Netherlands [n = 18], Germany [n = 9], Denmark[n = 3], and France [n = 1]) from 2008 to 2017 were subjected to whole-genomesequencing. An additional 25 draft genome assemblies of E. coli ST1250 and ST1250-SLV/DLV were obtained from the public databases. The isolates were compared forgenomic features, virulence genes, clade structure, and plasmid content. The completenucleotide sequences of eight IncHI1/ST9 plasmids and one IncHI1/ST2 plasmidwere obtained using long-read sequencing by PacBio or MinION. In the collection of79 isolates, only 10 were phylogenetically close (,8 single nucleotide polymorphisms[SNP]). The majority of isolates belonged to phylogroup B1 (73/79 [92.4%]) and carriedblaCTX-M-1 (58/79 [73.4%]). The plasmid content of the isolates was dominated byIncHI1 of ST9 (56/62 [90.3%]) and ST2 (6/62 [9.7%]), while 84.5% (49/58) of the blaCTX-M-1 genes were associated with the presence of the IncHI1 replicon of ST9 and 6.9% (4/58)with the IncHI1 replicon of ST2 within the corresponding isolates. The operon for theutilization of short-chain fructooligosaccharides (the fos operon) was present in 55 of 79(69.6%) isolates, and all of these carried IncHI1/ST9 plasmids. The eight completeIncHI1/ST9 plasmid sequences showed the presence of blaCTX-M-1 and the fos operonwithin the same molecule. Sequences of IncHI1/ST9 plasmids were highlyconserved (.98% similarity) regardless of country of origin and differed only inthe structure and integration site of the multidrug resistance (MDR) region.E. coli ST1250 and ST1250-SLV/DLV are phylogenetically diverse strains associatedwith horses. A strong linkage of E. coli ST1250 with the epidemic multidrugresistance plasmid lineage IncHI1/ST9 carrying blaCTX-M-1 and the fos operon wasidentified.
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anses-04018265 , version 1 (07-03-2023)

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Adam Valcek, Petra Sismova, Kristina Nesporova, Søren Overballe-Petersen, Ibrahim Bitar, et al.. Horsing Around: Escherichia coli ST1250 of Equine Origin Harboring Epidemic IncHI1/ST9 Plasmid with blaCTX-M-1 and an Operon for Short-Chain Fructooligosaccharide Metabolism. Antimicrobial Agents and Chemotherapy, 2021, 65 (5), pp.e02556-20. ⟨10.1128/aac.02556-20⟩. ⟨anses-04018265⟩

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