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Article Dans Une Revue Diseases of Aquatic Organisms Année : 2023

Epidemiological cut-off values for Vibrio anguillarum MIC and disc diffusion data generated by standardised methods

Edel Light
  • Fonction : Auteur

Résumé

This work aims to generate the data needed to set epidemiological cut-off values for minimum inhibitory concentration (MIC) and disc-diffusion zone measurements of Vibrio anguillarum. A total of 261 unique isolates were tested applying standard methods specifying incubation at 28 °C for 24-28h. Aggregated MIC distributions for a total of 247 isolates were determined in nine laboratories for eleven agents. Data aggregations of the disc zone for the ten agents analysed contained between 157 and 218 observations made by four to seven laboratories. Acceptable ranges for quality control (QC) reference strains were available for seven agents and the related multi-laboratory aggregated data were censored excluding the data of a laboratory that failed to meet QC requirements. Statistical methods were applied to calculate epidemiological cut-off values. Cut-off values for MIC data were calculated for florfenicol (≤1 µg/ml), gentamicin (≤4 µg/ml), oxytetracycline (≤0.25 µg/ml) and trimethoprim/sulfamethoxazole (≤0.125/2.38 µg/ml). The cut-off values for disc zone data were calculated for enrofloxacin (≥29 mm), florfenicol (≥27 mm), gentamicin (≥19 mm), oxolinic acid (≥24 mm), oxytetracycline (≥24 mm) and trimethoprim/sulfamethoxazole (≥26 mm). MIC and disc-diffusion zone data for the other agents where not supported by QC, thus yielding only provisional cut-off values (meropenem, ceftazidime). Regardless of whether QC is available or not, some of the aggregated MIC distributions (enrofloxacin, oxolinic acid), disc zone (sulfamethoxazole) and MIC and disc-diffusion distributions (ampicillin, chloramphenicol) did not meet the statistical requirements. The data produced will be submitted to CLSI for their consideration in setting international consensus epidemiological cut-off values.
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Licence : CC BY - Paternité

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anses-04311885 , version 1 (28-11-2023)

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Peter Smith, Laëtitia Le Devendec, Eric Jouy, Emeline Larvor, Alain Le Breton, et al.. Epidemiological cut-off values for Vibrio anguillarum MIC and disc diffusion data generated by standardised methods. Diseases of Aquatic Organisms, 2023, 155, pp.109 - 123. ⟨10.3354/dao03745⟩. ⟨anses-04311885⟩

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