European Inter-Laboratory Proficiency Test for Dourine Antibody Detection Using the Complement Fixation Test - Anses - Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail Accéder directement au contenu
Article Dans Une Revue Veterinary Sciences Année : 2023

European Inter-Laboratory Proficiency Test for Dourine Antibody Detection Using the Complement Fixation Test

Résumé

Dourine is a sexually transmitted parasitic disease affecting equids. Its causative agent is referred to as Trypanosoma equiperdum and the prescribed serodiagnosis method is the complement fixation test (CFT). In the context of our European Reference Laboratory mandate for equine diseases (excluding African horse sickness), we organised dourine CFT inter-laboratory proficiency tests (ILPTs) in 2015, 2018 and 2022 to evaluate the performance of the European Union network of National Reference Laboratories (NRLs) for dourine. ILPT panels were composed of horse sera with or without antibodies against Trypanosoma spp. originating from non-infected, immunised or experimentally infected horses. Twenty-two NRLs participated in at least one of the three sessions. In 2015, 2018 and 2022, the percentage of laboratories obtaining 100% of the expected results was 57, 90 and 80, respectively. These dourine CFT ILPTs showed the benefits of standardising the method’s detection limit and underlined the constant need to evaluate NRLs to improve the network’s performance. These results also argue in favour of the need for a representative bio-bank to improve the representativeness of ILPT samples and to allow the adoption of alternative serological methods for international surveillance of dourine.

Dates et versions

anses-04365581 , version 1 (28-12-2023)

Licence

Paternité

Identifiants

Citer

Laurent Hébert, Delphine Froger, Anthony Madeline, Fanny Lecouturier, Charlène Lemans, et al.. European Inter-Laboratory Proficiency Test for Dourine Antibody Detection Using the Complement Fixation Test. Veterinary Sciences, 2023, 10 (10), pp.592. ⟨10.3390/vetsci10100592⟩. ⟨anses-04365581⟩
35 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More