Development of a primary cell model derived from porcine dorsal soft palate for foot-and-mouth disease virus research and diagnosis. - Anses - Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail Accéder directement au contenu
Poster De Conférence Année : 2023

Development of a primary cell model derived from porcine dorsal soft palate for foot-and-mouth disease virus research and diagnosis.

Résumé

Foot-and-mouth disease virus (FMDV) persistence has been evidenced in dorsal soft palate (DSP) epithelial cells in ruminants. While the DSP is one of the primary sites of FMDV replication in both ruminants and swine, no persistent infectious virus was found in swine DSP. The lack of suitable in vitro models is an obstacle to knowledge progress regarding FMDV persistence. Although we have developed a bovine DSP cell model adapted to FMDV study, no model derived from porcine tissues of interest are currently available. Indeed, the porcine cells used are kidney cells such as IBRS-2. Primary cells were isolated from DSP collected from 6-month-old swine. These cells were then processed to isolate and maintain the epithelial cells. The presence of epithelial cell markers and FMDV-specific integrin receptors were assessed by immunofluorescence. Once characterised, DSP cells were infected in monolayers and multilayers grown at the air-liquid interface (ALI) with a type-O FMDV. Cell culture supernatant were regularly collected until 115 dpi and were tested for the presence of infectious virus, viral RNA and viral antigen. Analysis of supernatants from the multi-layered model infection led to the detection of infectious virus up to 7 dpi, as well as viral RNA up to 23 dpi. Consistent with in vivo observations, no evidence of FMDV persistence was observed in our model as no infectious viruses were detected after 28 dpi. The relevance of this model having been confirmed by our results, it would be considered to immortalise these cells in view of developing a FMDV-sensitive porcine epithelial cells lineage. Regarding their sensitivity to a wide diversity of reference and field FMDV strains, as well as other viruses such as Vesicular Stomatitis Virus, Seneca Valley Virus and Swine Vesicular Disease Virus, we consider these cells as a very promising tool for FMDV research and differential diagnosis.
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Dates et versions

anses-04425022 , version 1 (29-01-2024)

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Paternité - Pas d'utilisation commerciale - Pas de modification

Identifiants

  • HAL Id : anses-04425022 , version 1

Citer

Morgan Sarry, Cindy Bernelin-Cottet, Caroline Michaud, Anthony Relmy, Aurore Romey, et al.. Development of a primary cell model derived from porcine dorsal soft palate for foot-and-mouth disease virus research and diagnosis.. Global Foot-and-Mouth (F M D) Research Alliance (G F R A), Nov 2023, Kampala, Uganda. . ⟨anses-04425022⟩
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