RAPID, ON SITE, DIAGNOSIS OF FMD AND SAFE AND COST-EFFECTIVE SHIPMENT OF SAMPLES USING LATERAL FLOW DEVICES FOR LABORATORY DIAGNOSTIC
Résumé
Introduction:
Foot-and-mouth disease (FMD), caused by infection with foot-and–mouth disease virus (FMDV), is one of the most economically devastating diseases affecting artiodactyls. Identification of circulating strains is important for efficient FMD control. However, due to shipping requirements of potentially infected samples, the cost of sample submission to reference laboratories remains a major obstacle. A cost-effective and safe method for shipment of samples from FMD-suspected cases, based on the inactivation of FMDV on lateral flow devices (LFDs) has been developed and validated in the laboratory which allows subsequent detection and typing of FMDV by RT-PCR and virus rescue using RNA transfection (Romey et al. 2017). The present study aims to further evaluate this protocol on freshly collected clinical samples through collaboration in endemic countries in order to test the performance and safety of the entire process directly in the field.
Materials-methods:
Epithelium or vesicular fluid samples will be collected from suspect clinical cases of FMD in Nigeria, Turkey and Pakistan and will be tested in the field using LFDs. The selected positive inactivated (or not) LFDs will be submitted to reference laboratories (France, Denmark) for molecular detection and virus rescue.
Results:
Sample collection and inactivation on LFDs are in progress. Transfections are currently being optimized to ensure virus rescue from RNA genomes recovered from inactivated LFDs.
Discussion-Conclusions:
This study will contribute to demonstrate that using LFDs is a safe way for room-temperature, dry-transport of inactivated FMDV samples from endemic areas. It will substantially decrease the shipping cost thus increasing field sample submission.
Domaines
Sciences du Vivant [q-bio]Origine | Fichiers produits par l'(les) auteur(s) |
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