Gene signatures associated with foot-and-mouth disease virus infection and persistence. Part II: Transcriptomic analysis - Anses - Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail Accéder directement au contenu
Communication Dans Un Congrès Année : 2018

Gene signatures associated with foot-and-mouth disease virus infection and persistence. Part II: Transcriptomic analysis

Eve Laloy
Aurore Romey
Anthony Relmy
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Kamila Gorna
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Stéphan Zientara
Labib Bakkali Kassimi
Sandra Blaise-Boisseau

Résumé

Foot-and-mouth disease virus (FMDV; Picornaviridae) causes devastating disease in livestock. Following acute infection, FMDV can persist in the soft palate of ruminants, but the persistence mechanisms remain unknown. To identify gene expression signatures that can be used to prevent or diagnose persistent infections, cells from an air-liquid interface multilayer model of bovine soft palate were infected with FMDV. The experiment was performed twice with different donor animals and two biological replicates per timepoint (0, 24 hours and 28 days after infection). Protein and RNA were extracted, whole-transcriptome libraries were prepared and sequenced with an Ion S5XL in two independent runs (median reads/sample ~24 million). Sequence data were analysed with Salmon and DESeq2 to detect differential expression between infected and non-infected cells at 24 hours (acute) and 28 days (persistent infection), as well as between 24 hours and 28 days in infected cells. Selected genes were confirmed by RT-qPCR and protein mass spectrometry. Animals, treatments and timepoints (in the infected cells) were clearly separated in the principal component analysis. During acute infection, 423 genes were differentially expressed (406 up, 17 down), with the upregulated genes consistent with responses to viral infections: e.g., MX2, IFIT2, OAS2, IFI27 and MX1. A reduced set of these genes (62) was upregulated at 28 days, coinciding with lower levels of FMDV transcripts than during acute infection. Uniquely, genes of keratinocyte differentiation and extracellular matrix organisation (e.g., SPRR3, NCAM1, ADAMTS16 and collagens) were downregulated at that time, indicating a modulation of epithelial maturation during persistent FMDV infection.
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Dates et versions

anses-04441809 , version 1 (06-02-2024)

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  • HAL Id : anses-04441809 , version 1

Citer

Florian Pfaff, Sara Hägglund, Eve Laloy, Aurore Romey, Anthony Relmy, et al.. Gene signatures associated with foot-and-mouth disease virus infection and persistence. Part II: Transcriptomic analysis. RNA VIRUS PERSISTENCE MEETING: MECHANISMS AND CONSEQUENCES, Aug 2018, Freiburg, Germany. ⟨anses-04441809⟩
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