GENE SIGNATURES ASSOCIATED WITH FOOT-AND-MOUTH DISEASE VIRUS INFECTION AND PERSISTENCE PART II: TRANSCRIPTOMIC ANALYSIS OF ACUTE AND PERSISTENT FMDV INFECTION IN BOVINE SOFT PALATE - Anses - Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail Accéder directement au contenu
Communication Dans Un Congrès Année : 2018

GENE SIGNATURES ASSOCIATED WITH FOOT-AND-MOUTH DISEASE VIRUS INFECTION AND PERSISTENCE PART II: TRANSCRIPTOMIC ANALYSIS OF ACUTE AND PERSISTENT FMDV INFECTION IN BOVINE SOFT PALATE

Sandra Blaise-Boisseau
Eve Laloy
Labib Bakkali Kassimi
Aurore Romey
Anthony Relmy
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Kamila Gorna
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Stéphan Zientara

Résumé

Introduction The transcriptional response of the nasopharynx and soft palate (SP) during FMDV infection in cattle is not well understood. Therefore, an air-liquid interface multilayer model of bovine SP cells was developed and is presented in a joint publication. In combination with RNA sequencing, this novel system was used to identify host gene signatures during acute (24 hours post infection - HPI) and persistent infection (28 days post infection - DPI) in order to determine mechanisms and factors that may help to prevent persistent infection or to improve diagnostics. Materials and methods The infection experiment was performed twice with different donor animals and two biological replicates per animal and timepoint (0, 24 HPI and 28 DPI). Whole-transcriptome libraries were sequenced with the Ion S5XL (reads/replicate: ~24 million) and the gene expression was analyzed using Salmon and DESeq2. Expression levels of selected genes and FMDV genome were confirmed by RT-qPCR and protein mass spectrometry. Results Principal component analysis demonstrated the robustness of the RNA sequencing, showing strong influence of donor animal, infection and timepoint. 315 and 73 genes were differentially expressed at 24 HPI and 28 DPI, respectively. The majority of differentially expressed genes were up-regulated and related to the immune system (MX1, OAS2, IFIH1). Interestingly, 10 genes were only regulated during persistent infection (ANKRD1, NCAM1, collagens). Most of these were down-regulated and related to the development of the extracellular matrix or keratinocyte differentiation. Discussion The results indicate time-dependent gene signatures during FMDV infection that include the activation of the innate immune system, particularly interferon and cytokine signaling. However, the overall number of regulated genes and their expression was reduced 28 DPI, in comparison to acute infection. Furthermore, the down-regulation of a few unique genes 28 DPI indicates a modulation of epithelial maturation during persistent FMDV infection. These genes represent interesting candidates for future experiments.
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anses-04442182 , version 1 (06-02-2024)

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  • HAL Id : anses-04442182 , version 1

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Florian Pfaff, Sara Hägglund, Sandra Blaise-Boisseau, Eve Laloy, Labib Bakkali Kassimi, et al.. GENE SIGNATURES ASSOCIATED WITH FOOT-AND-MOUTH DISEASE VIRUS INFECTION AND PERSISTENCE PART II: TRANSCRIPTOMIC ANALYSIS OF ACUTE AND PERSISTENT FMDV INFECTION IN BOVINE SOFT PALATE. EuFMD Open Session 2018, EuFMD, Oct 2018, Borgo-Egnazia, Italy. ⟨anses-04442182⟩
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