Change in the molecular properties of CH1641 prions after transmission to wild‐type mice: Evidence for a single strain - Anses - Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail Accéder directement au contenu
Article Dans Une Revue Neuropathology and Applied Neurobiology Année : 2024

Change in the molecular properties of CH1641 prions after transmission to wild‐type mice: Evidence for a single strain

Résumé

Aim: CH1641 was discovered in 1970 as a scrapie isolate that was unlike all other classical strains of scrapie isolated so far. We performed bio-assays of CH1641 in mice in order to further characterise this specific isolate. Methods: We inoculated the original CH1641 isolate into ovine and bovine prion protein (PrP) transgenic mice as well as wild-type mice. In addition, we performed cross-and back passages between the various mouse lines to examine if one identical prion strain was isolated in all mouse lines or whether multiple prion strains exist in CH1641. Results: We report the first successful transmission of CH1641 to wild-type RIII mice and via RIII mice to wild-type VM mice. Unexpectedly, analysis of the protease-resistant prion protein (PrP res) in wild-type mice showed a classical scrapie banding pattern differing from the banding pattern of the original CH1641 isolate. Cross-and back passages of CH1641 between the various mouse lines confirmed that the same prion strain had been isolated in all mouse lines. Conclusions: The CH1641 isolate consists of a single prion strain but its molecular banding pattern of PrP res differs between wild-type mice and PrP transgenic mice. Consequently, molecular banding patterns of PrP res should be used with caution in strain typing since they do not solely depend on the properties of the prion strain but also on the host prion protein.
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anses-04459932 , version 1 (15-02-2024)

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Lucien J M van Keulen, Corry H Dolstra, Ruth Bossers‐de Vries, Alex Bossers, Jorg G Jacobs, et al.. Change in the molecular properties of CH1641 prions after transmission to wild‐type mice: Evidence for a single strain. Neuropathology and Applied Neurobiology, 2024, 50 (1), ⟨10.1111/nan.12963⟩. ⟨anses-04459932⟩

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