Gene Signatures Associated with Foot-And-Mouth Disease Virus Infection and Persistence Part II: Proteogenomics Uncovers Critical Elements of Host Response in Bovine Soft Palate Cells - Anses - Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail Accéder directement au contenu
Communication Dans Un Congrès Année : 2019

Gene Signatures Associated with Foot-And-Mouth Disease Virus Infection and Persistence Part II: Proteogenomics Uncovers Critical Elements of Host Response in Bovine Soft Palate Cells

Résumé

Foot-and-mouth disease (FMD) is the most devastating disease of cloven-hoofed livestock, with a crippling economic burden in endemic areas and immense costs associated with outbreaks in free countries. Foot-and-mouth disease virus (FMDV), a picornavirus, will spread rapidly in naïve populations, reaching morbidity rates of up to 100% in cattle. Even after recovery, over 50% of cattle remain subclinically infected and infectious virus can be recovered from the nasopharynx. The transcriptional alterations of the nasopharynx and soft palate (SP) during FMDV infection and persistence in cattle are not well understood. Here, an air-liquid interface multilayer model of bovine SP cells was used to study the hosts transcriptional signatures during acute (24 hours post infection - HPI) and persistent FMDV infection (28 days post infection - DPI). Therefore, whole-transcriptome libraries were sequenced with the Ion S5XL. Furthermore, expression levels of selected genes were confirmed by RT-qPCR and protein mass spectrometry. During the acute phase viral RNA and protein was detectable in large quantities and in response hundreds of interferon-stimulated genes (ISG) were overexpressed, mediating antiviral activity and apoptosis. Although the number of pro-apoptotic ISGs and the extent of their regulation decreased during persistence, some ISGs with antiviral activity were still highly expressed at that stage. This indicates a long-lasting but ultimately ineffective stimulation of ISGs (MX1, OAS2, IFIH1) during FMDV persistence. Furthermore, downregulation of relevant genes (ANKRD1, NCAM1, collagens) suggests an interference with the extracellular matrix that may contribute to the skewed virus-host equilibrium in soft palate epithelial cells.

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Dates et versions

anses-04513975 , version 1 (20-03-2024)

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Paternité - Pas d'utilisation commerciale - Pas de modification

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  • HAL Id : anses-04513975 , version 1

Citer

Florian Pfaff, Sara Hägglund, Martina Zoli, Sandra Blaise-Boisseau, Eve Laloy, et al.. Gene Signatures Associated with Foot-And-Mouth Disease Virus Infection and Persistence Part II: Proteogenomics Uncovers Critical Elements of Host Response in Bovine Soft Palate Cells. Epizone 13th annual meeting, Epizone, Aug 2019, Berlin (Germany), France. ⟨anses-04513975⟩
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