RAPID, ON SITE, DIAGNOSIS OF FMD AND SAFE AND COST-EFFECTIVE SHIPMENT OF SAMPLES - Anses - Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail Accéder directement au contenu
Communication Dans Un Congrès Année : 2019

RAPID, ON SITE, DIAGNOSIS OF FMD AND SAFE AND COST-EFFECTIVE SHIPMENT OF SAMPLES

Aurore Romey
Labib Bakkali Kassimi
Graham J. Belsham
  • Fonction : Auteur
  • PersonId : 1019759
Abdulnaci Bulut
  • Fonction : Auteur
Syed Jamal
Eve Laloy
Anthony Relmy
  • Fonction : Auteur
  • PersonId : 1075108
Stéphan Zientara
Sandra Blaise-Boisseau

Résumé

Foot-and–mouth disease virus (FMDV) causes one of the most economically devastating animal diseases affecting artiodactyls. Identification of circulating strains is important for efficient FMD control. However, shipping requirements and high cost of submission of suspected positives samples to reference laboratories remain a major obstacle. A cost-effective and safe method for shipment of samples from FMD-suspected cases, based on the inactivation of FMDV on lateral flow devices (LFDs, immunodetection strips) has been developed and validated in the laboratory. This method allows detection and typing of FMDV by RT-PCR and virus rescue using RNA transfection (Romey et al. 2017). The present study aims to further evaluate this protocol on freshly collected clinical samples through collaborations with endemic countries in order to test performance and safety of the entire process directly in the field. Epithelium or vesicular fluid samples have been collected from suspect clinical cases of FMD in Nigeria, Turkey and Pakistan and have been tested in the field using LFDs. The selected positive inactivated (or not) LFDs have been submitted to reference laboratories (France, Denmark) for molecular detection and virus rescue. Safety of this inactivation protocol has been confirmed directly in Nigerian and Turkish laboratories. Transfections are currently being optimized to ensure virus rescue from RNA genomes recovered from inactivated LFDs. This study will contribute to demonstrate that using LFDs is a safe way for room-temperature, dry-transport of inactivated FMDV samples from endemic areas. It will substantially decrease the shipping cost thus increasing field sample submission.
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anses-04514003 , version 1 (20-03-2024)

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Paternité - Pas d'utilisation commerciale - Pas de modification

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  • HAL Id : anses-04514003 , version 1

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Aurore Romey, Labib Bakkali Kassimi, Graham J. Belsham, Abdulnaci Bulut, Claude Hamers, et al.. RAPID, ON SITE, DIAGNOSIS OF FMD AND SAFE AND COST-EFFECTIVE SHIPMENT OF SAMPLES. Epizone 13th annual meeting, Epizone, Aug 2019, Berlin (Germany), France. ⟨anses-04514003⟩
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