Field evaluation of a safe and cost-effective shipment of FMD suspected samples to diagnostic laboratories using lateral flow devices - Anses - Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail Accéder directement au contenu
Communication Dans Un Congrès Année : 2021

Field evaluation of a safe and cost-effective shipment of FMD suspected samples to diagnostic laboratories using lateral flow devices

Graham J. Belsham
  • Fonction : Auteur
  • PersonId : 1019759
Souheyla Benfrid
Cindy Bernelin-Cottet
  • Fonction : Auteur
Abdulnaci Bulut
  • Fonction : Auteur
Michael Eschbaumer
  • Fonction : Auteur
  • PersonId : 1006303
Claude Hamers
  • Fonction : Auteur
Syed Jamal
Anthony Relmy
  • Fonction : Auteur
  • PersonId : 1075108
Aurore Romey
Hussaini Ularamu
Yiltawe Wungak
  • Fonction : Auteur
Stéphan Zientara
Sandra Blaise-Boisseau

Résumé

Foot-and-mouth disease virus (FMDV) causes a highly contagious vesicular disease in livestock with serious consequences for international trade. The control of FMD requires the identification of circulating strains in affected areas to implement effective control measures. However, restrictive regulations and high costs for shipping of samples to reference laboratories remain major obstacles. In our laboratory, we developed a cost-effective and safe protocol for shipment of FMDV samples based on the inactivation of the virus on lateral flow devices (LFD). This method allows molecular detection and typing of FMDV and live virus rescue after RNA transfection. The present study further evaluates this protocol on clinical samples freshly collected in endemic countries. In Nigeria, Turkey and Pakistan, epithelium or vesicular fluid samples were collected on FMD-suspected clinical cases and tested in the field for FMD using LFD. LFDs were inactivated or not, and submitted to reference laboratories for further molecular detection and virus rescue. In parallel, the protocol was tested on vesicular fluid collected from cattle experimentally infected with FMDV. The safety of the inactivation protocol was confirmed on field samples, as well as on vesicular fluid collected from experimentally infected cattle. On inactivated LFDs, molecular diagnosis was successfully performed and transfection ensured virus rescue for some samples. This study demonstrates that this LFDs-based inactivation protocol is a safe way for room-temperature, dry-transportation of FMDV samples. It will substantially decrease the shipping cost, thus increasing field sample submission to contribute to a better FMD control.
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Dates et versions

anses-04523885 , version 1 (27-03-2024)

Identifiants

  • HAL Id : anses-04523885 , version 1

Citer

Labib Bakkali Kassimi, Graham J. Belsham, Souheyla Benfrid, Cindy Bernelin-Cottet, Abdulnaci Bulut, et al.. Field evaluation of a safe and cost-effective shipment of FMD suspected samples to diagnostic laboratories using lateral flow devices. EAVLD Virtual Meeting 2021, European Association of Veterinary Laboratory Diagnosticians, Nov 2021, on-line meeting, France. ⟨anses-04523885⟩
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