In vitro toxicity of polyethylene terephthalate nanoplastics (PET-NPs) in human hepatocarcinoma (HepG2) cell line - Anses - Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail Accéder directement au contenu
Article Dans Une Revue Toxicology and Environmental Health Sciences Année : 2024

In vitro toxicity of polyethylene terephthalate nanoplastics (PET-NPs) in human hepatocarcinoma (HepG2) cell line

Résumé

Objective: Nanoplastics (NPs) are consider as emerging persistent environmental pollutants. Widespread distribution of these nanoparticles is a global problem. However, their toxic effects in mammalian tissues and cells remain mainly unknown. This study aims to investigate the cytotoxicity of PET nanoplastics (PET-NPs) in the human hepatocarcinoma (HepG2) cell line. Methods: Toxic effects after 72h of exposure to different concentrations of PET-NPs (10–500 µg/mL) were evaluated by morphological alterations, cell internalization, cell viability (MTT), lactate dehydrogenase (LDH) release assays, induction of oxidative stress (total antioxidant capacity, TAC), and genotoxicity (comet assay). Results: Cell viability reduced at all treatment concentrations in a dose–response manner, and 616.7 µg/mL was determined as IC50. No cell membrane damages detected by LDH assay. TAC reduced significantly after 12 h exposure to > 400 μg/mL PET-NPs. Dose-dependent DNA damages were observed after 72 h. Conclusion: These findings indicated that PET-NPs have significant cytotoxic effects, particularly on genotoxicity and induction of oxidative stress. The results obtained here showed a significant impact of PET-NPs at the tested concentrations suggest a potential impact on humans. Other studies are currently underway to confirm these toxic effects.
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Dates et versions

anses-04661173 , version 1 (24-07-2024)

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Zahra Manoochehri, Mahmoud Etebari, Pauline Pannetier, Karim Ebrahimpour. In vitro toxicity of polyethylene terephthalate nanoplastics (PET-NPs) in human hepatocarcinoma (HepG2) cell line. Toxicology and Environmental Health Sciences, 2024, 16 (2), pp.203-215. ⟨10.1007/s13530-024-00213-z⟩. ⟨anses-04661173⟩

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