Article Dans Une Revue Journal of Global Antimicrobial Resistance Année : 2025

No genetic link between E. coli isolates carrying mcr-1 in bovines and humans in France

Résumé

Background: Colistin is a last-line antibiotic used to treat severe human infections caused by carbapenemase-producing Gram-negative bacteria. In parallel, colistin has massively been used in the veterinary field so that mcr-1 -positive E. coli have spread worldwide in livestock, potentially constituting a reservoir of colistin-resistant isolates that can be further transmitted to humans. Objectives: In France, the mcr-1 gene was frequently identified in E. coli of bovine origin. This genomic study assessed whether French human mcr-1 -positive E. coli might originate or derive from the bovine reservoir. Material and methods: Human (n = 24) and bovine (n = 127) isolates collected between 2011 and 2019 were included and colistin-resistance was confirmed by MICs. The detection of mcr-1 was performed by PCR. Isolates were short-read whole-genome sequenced and a cgMLST-based phylogeny was constructed. The genetic support of mcr-1 was identified using short-read sequences or Southern blots. Results: The mcr-1 gene was carried by a high diversity of genetic backgrounds, among which ST167 and ST10 were the most widespread. No clonally-related isolates between bovines and humans were observed. In bovines, mcr-1 was identified on IncHI2 and IncX4 plasmids and increasingly on the chromosome, while it was also found on IncI2 and p0111 plasmids in humans.
Conclusion:

Although similar STs (ST744 and ST88) and plasmid types (IncHI2, IncX4) carried mcr-1 , no hypothesis of a transfer from bovines to humans could be supported by the data. Furthermore, the increasing chromosomal location of mcr-1 over time may reflect an animal-specific evolutionary pathway deserving further investigation.

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Bactériologie
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anses-04894989 , version 1 (17-01-2025)

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Marisa Haenni, Pierre Châtre, Racha Beyrouthy, Antoine Drapeau, Pauline François, et al.. No genetic link between E. coli isolates carrying mcr-1 in bovines and humans in France. Journal of Global Antimicrobial Resistance, 2025, 41, pp.111-116. ⟨10.1016/j.jgar.2024.12.021⟩. ⟨anses-04894989⟩
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