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Poster De Conférence Année : 2021

Field evaluation of a safe and cost-effective shipment of suspected samples to diagnostic laboratories using lateral flow devices

Aurore Romey
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Souheyla Benfrid
Abdulnaci Bulut
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Michael Eschbaumer
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  • PersonId : 1006303
Syed Jamal
Anthony Relmy
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  • PersonId : 1075108
Stéphan Zientara
Sandra Blaise-Boisseau

Résumé

The control of Foot-and-mouth disease (FMD) requires the identification of circulating strains in endemic areas. However, shipping requirements and high cost for submission of suspected positive samples to reference laboratories remain major obstacles. We developed in our laboratory a cost-effective and safe method for shipment of samples from FMD-suspected cases, based on the inactivation of FMDV on lateral flow devices (LFDs) relying on immunodetection strips. This method allows detection and typing of FMDV by RT-PCR and virus rescue after RNA transfection (Romey et al. 2018). The present study further evaluates this protocol on clinical samples freshly collected in endemic settings through collaborations to test the performances and safety of the entire process. In Nigeria, Turkey and Pakistan, epithelium or vesicular fluid samples were collected on FMD-suspected clinical cases and tested in the field for FMD using LFDs. Half of the positive LFDs were inactivated and all positive immunodetection strips were submitted to reference laboratories for further molecular detection and virus rescue. In parallel, the protocol was tested in a similar manner on vesicular fluid collected from cattle experimentally infected with FMDV. The safety of this inactivation protocol has been confirmed on field samples directly in Nigerian and Turkish laboratories, as well as on vesicular fluid collected from experimentally infected cattle at FLI. On inactivated LFDs, molecular diagnosis was successfully performed and transfection ensured virus rescue for some samples leading to RNA full-genome sequencing. This study demonstrates that this LFDs-based inactivation protocol is a safe way for room-temperature, dry-transportation of FMDV samples from endemic areas. It will substantially decrease the shipping cost, thus increasing field sample submission to contribute to a better FMD control.
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Dates et versions

anses-04526323 , version 1 (29-03-2024)

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Paternité - Pas d'utilisation commerciale - Pas de modification

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  • HAL Id : anses-04526323 , version 1

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Aurore Romey, Graham J. Belsham, Souheyla Benfrid, Cindy Bernelin-Cottet, Abdulnaci Bulut, et al.. Field evaluation of a safe and cost-effective shipment of suspected samples to diagnostic laboratories using lateral flow devices. Global Foot-and-Mouth (F M D) Research Alliance (G F R A), Nov 2021, Virtual Conference, Argentina. ⟨anses-04526323⟩
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